multiplex assay (rus. мультиплексный анализ) — a type of medical and biological analytical procedures that simultaneously measure multiple complex molecules in a single biological sample.

Description

A multiplex assay is used to detect different biomolecules (from 10 to 100) of a given class (DNA, RNA or proteins) in a biological sample. Depending on the type of molecules to be detected, the assay involves the use of certain sets of nucleic acids or proteins. For example, a multiplex immunoassay involves the use of latex microspheres; each pre-coated with a unique antibody and coded with a specific combination of fluorescent quantum dots. This method allows detection of between 10 and 100 biological markers in a drop of a patient’s blood in several minutes.

Illustrations

Multiplex immunology analysis of antibody-based biomarkers conjugated with CdTe fluorescent qua
Multiplex immunology analysis of antibody-based biomarkers conjugated with CdTe fluorescent quantum dots. The proposed analysis example involves a competitive reaction of specific antibodies with antigens in a test specimen and on a standard substrate. If the antigen is present in the specimen, antibodies bind with the substrate less intensively, leading to a proportionate reduction of fluorescence of corresponding quantum dots settled on the substrate.

Author

  • Borisenko Grigory G.

Source

  1. Wang L. et al. Nanoparticles for multiplex diagnostics and imaging // Nanomedicine. 2006. V. 1, №4. P. 413–26.

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